In the laboratories of the Jagiellonian Center of Innovation, microbiological analyses of biological, pharmaceutical and environmental materials are conducted. The diagnostics is performed using a conventional method (smear on a substrate, culture, microscopic analysis) and a new diagnostic method using MALDI-TOF mass spectrometry.
Staining and microscopic assessment of specimens:
- automatic specimen staining using Gram's method: automated staining instrument PreviColour (bioMerieux)
- microscopic assessment of microbiological specimens: microscopic microorganism identification, assessment of the degree of material purity using a simple biological microscope Eclipse Ni-U with a camera and a video camcorder (Nikon)
- smear test: automatic dilution series 5 x 1/10 and automatic smear on a Petri dish, with a countable range from 30 to cfu/mL.: Spiral Dilute instrument (Interscience)
- automatic counting of colonies and growth inhibition zones as per EUCAST (European Committee on Antimicrobial Susceptibility Testing), along with statistical analysis of the results: Scan 1200 automatic HD colony Counter (Interscience)
- microscopic photographs of the grown microorganisms: microphotography stand (Kaiser)
Identification of microorganisms using a MALDI TOF mass spectrometer:
- identification of bacteria, yeast-like fungi, filamentous fungi and mold fungi performed using MALDI TOF mass spectrometry with the use of matrix-assisted laser desorption/ionization with time-of-flight analyzer: MALDI TOF mass spectrometer, Microflex LT, Maldi Biotyper (Bruker)
Identification of a microorganism is performed over a few minutes, as compared to 24–48 hours necessary to identify the microorganism using conventional methods. A protein set, the so-called “molecular fingerprint” of the microorganism is compared to protein profiles of reference strain, thus allowing for the identification of the studied strain. A single colony is sufficient to perform the study.