Cell cultures are performed in the Laboratory for Tissue and Cell Culture of the Jagiellonian Center of Innovation, in cleanrooms, as per GMP standards. The rooms have been adapted for culturing human tissues and cells for further clinical use.
Constant monitoring of room parameters, as well as laboratory instruments, while maintaining sterile working conditions guarantees safe work with materials of human origin, with respect to both the biological material prepared to be used as graft and personnel safety. This enables treating patients using the state-of-the-art techniques and the most effective treatment methods, including transplant of autologous cells grown in vitro.
The Tissue Bank allows for material storage to ensure a necessary amount of the material required for transplant in the case when the procedure must be repeated.
The Laboratory for Tissue and Cell Culture offers cooperation within the scope of:
- isolation and culture of stem cells from heterogeneous population, including those from bone marrow, umbilical cord blood, Wharton's jelly, mobilized peripheral blood and other mature tissues
- preparation of the obtained cells for autologous application
- cell banking and storage
The cell tests are performed with the use of eukaryotic cells. The most standard methods analyze the cytotoxicity of external, physical and chemical factors towards the cell biology. To obtain this goal, the following tests are performed: MTT, MTS and LDH after the selection of an appropriate cell model and based on the recommended standards.
MTT and MTS tests allow for the measurement of mitochondrial enzyme activity in a cell. Mitochondrial oxidative activity is proportional to the amount of yellow tetrazole MTT or MTS salt reduced to blue formazan (blue coloring can be measured spectrophotometrically). The difference between the tests is as follows: use of MTT, in contrast to MTS, results in an insoluble product that must be suspended in an organic solvent. In strictly defined experimental conditions, these tests may be used to determine the viability of non-dividing but metabolically active cells.
Lactate dehydrogenase (LDH) is an intracellular enzyme. Mechanical damage to a plasma membrane and cell death caused by external activity result in LDH release from the cells to the environment. Assaying LDH activity in the supernatant provides the measurement of toxicity of the studied substance against the cells in the culture. Enzymatic reactions used in the described method occur in two steps, finally resulting in a colored product that is assayed spectrophotometrically.
We also offer our cooperation within the scope of research and obtaining funds and research grants.
Agnieszka Witulska, quality assurance & control manager/responsible person
T: +48 12 297 46 42 E: firstname.lastname@example.org